How much bsa to add to restriction digest

Protein Marker. PCR-Reaction Plates. PV-Energy Production. GeneON Bioscience. Get newsletter from GeneON with special offers! English pages Enzymes - Fine-Chemicals: Agarose. BSA prevent the adhesion of enzymes at reaction tubes.

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Read our cookie policy. Restriction enzyme overview. Find a restriction enzyme. Traditional molecular cloning Restriction enzyme overview General information about restriction enzymes Star activity of restriction enzymes Inactivation of restriction enzymes Buffer activity with restriction enzymes Universal buffers for double digestion with restriction enzymes Restriction enzymes affected by methylation Methylation-sensitive restriction enzymes QC of restriction enzymes Ligation cloning overview Ligation product guide.

New products. Contact Sales. Universal buffers for double digestion with restriction enzymes Double digestion digesting DNA with two restriction enzymes simultaneously is frequently performed to save time.

DNA may not be digested completely when recognition sequences of two enzymes are close each other for DNAs with high-structure conformations. Your partner for molecular diagnostic development and supply As an experienced, reliable solutions provider, we offer full-scale customization services for a vast array of reagents and instruments backed by a quality management system, on-time delivery, and dependable support—giving you a competitive advantage in the ever-changing clinical landscape.

Stem cell services Clinical-grade stem cell services Research-grade stem cell services Outsourcing stem cell-based disease model development Gene and cell therapy manufacturing services Services Facilities Our process Resources. Some restriction enzymes require high salt and CutSmart Buffer is a low salt buffer. Over restriction enzymes are supplied with CutSmart Buffer, but the remaining enzymes performed more optimally in another buffer.

Please refer to the Performance Chart. How do I perform a double digest with an enzyme that comes with the new buffer and an enzyme that comes with the old buffer system?

We recommend using the Double Digest Finder and use the buffer it recommends. Alternatively, if it recommends CutSmart Buffer, then you can simply use the new buffer.

My restriction enzyme used to be available at a lower concentration. This contributes to ease of use and often results in improved performance by having less glycerol in the digest. Why did you add BSA into all the restriction enzyme reaction buffers?

As an added convenience for our customers, NEB has included BSA in all its restriction enzyme buffers, eliminating the need to add it as a separate step. This makes digestion reactions simpler, set-up time even faster, and decreases the number of tubes to store. Why did you remove DTT from your restriction enzyme buffers? DTT was originally included in the buffer formulation for historical reasons.